Effect of basal medium on growth and polyphenols accumulation by Gardenia jasminoides Ellis cell suspension
نویسندگان
چکیده
The goal of this study was to analyze the effect different medium bases on accumulation biomass and secondary metabolites production by Gardenia jasminoides Ellis. cell suspension culture. result revealed that media have significant polyphenol compounds. HPLC analyses showed higher content achieved when cells were cultivated McCOWN Woody plant Medium (WP) for 14 days darkness, at 24°C. At conditions, produced maximal amounts catechin (24.44±2.46 µg/g DW), chlorogenic acid (20.26 ±3.68 epicatechin (70.44±7.46 DW) rutin (45.90±0.26 DW). Both MS WP optimal accumulation. When these media, accumulated amount dry ADB = 0.99±0.1g/100ml 0.93±0.04 g/100ml МS WP, respectively. corresponding growth indexes GIDW 1.68±0.2 1.58±0.07 medium. reported results are first step future optimization nutrient composition cultivation conditions essential scale -up process G. culture mass polyphenolic
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ژورنال
عنوان ژورنال: BIO web of conferences
سال: 2022
ISSN: ['2273-1709', '2117-4458']
DOI: https://doi.org/10.1051/bioconf/20224502006